Four BRF jars (see PF tek) inoculated with around 2mL live fragmented Golden Oyster (Pleurotus citrinopileatus) liquid culture. 370mL jars used (Bonne Maman jam).
First Signs of Colonization - 6 days post-inoculation
Initial wispy signs of colonization - 6 days post-inoculation
Initial signs of colonization - 6 days post-inoculation
8 days post-inoculation
10 days post-inoculation showing metabolite formation (brown spot)
Jars fully colonized after 14 days. Small quantities of metabolites observed in 2/4 jars (see above image). Cakes birthed and spawned to straw (see Straw tek).
Mycelium breaking through top layer of straw - 2 days post-spawn
Signs of mycellial breakthrough - 2 days post-spawn
After 7 days, basket looks almost 100% colonized.
Full colonization of substrate - 7 days post-spawn
Surface colonization - 7 days post-spawn
Colonization of basket side - 7 days post-spawn
After 13 days post-spawn (6 days after basket was fully colonized), I moved the basket into a shotgun FC. It was when I did this, that I discovered the first pinsets had developed. The basket was pinning on all sides, including a large pinset that had erupted from the bottom of the basket, through one of the drainage holes.
Fully colonized basket in shotgun FC
Lid left partially off FC, with misting 2-3 times per day. Humidity remaining around 70-75%. Temperature around 15C (60F).
Primordia formation - day 13
Primordia starting to form individual “heads”, which will (hopefully!) become mushrooms. I’ve noticed that some pinheads have stalled. These will possibly continue to develop after the first harvest.
Primordia development - day 15
Primordia development - day 16
Maturing fruitbodies - day 18
Maturing fruitbodies - day 19
Near mature fruitbodies - day 20
First flush harvest - day 21