200mL distilled water
8mL organic clear liquid honey
spore solution or liquid mycellium culture
Punch two holes in the lid of your jar, and put a small piece of tube in one hole, and seal it with silicone. Fill this tube with polyfill (the stuffing from polyester pillows and soft-toys). Across the other hole, put a blob of silicone and smooth it over somewhat. Wait for silicone to completely dry (at least 2 hours).
Make sure your jar is clean. Put the marble in the jar, add 200mL distilled water to it and add your 8mL honey. If you want to make more or less liquid culture (LC), then just use the same ratio of water to honey (96% water, 4% honey). Give the honey/water a good mix until all of the honey is dissolved. Screw the lid you just made onto the jar, and pressure cook it for 15-20min. Leave the jar to cool inside the pressure cooker overnight (or until it is completely cool/room temperature). It is now ready to inoculate with your LC or spore solution.
Inside your laminar flowhood or glovebox (see Glovebox tek), wipe the silicone injection port with 70% ethanol or isopropanol. Take your syringe with a sterile needle attached, and inject through the port. Inoculate with 1mL spore solution or LC. When you remove the needle, the silicone will self heal. If you wish, you can put a strip of micropore tape over the port, although if your port was constructed well, this is unnecessary.
Put the inoculated jar in a dark, warm place. Once a day, have a look at the jar and give it a swirl. The liquid culture should stay completely clear (like it was after it came out of the PC). You’ll notice clumps of fluffy white material starting to form over time.